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recombinant proteins m csf sinobiological  (Sino Biological)


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    Sino Biological recombinant proteins m csf sinobiological
    Recombinant Proteins M Csf Sinobiological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csf+sinobiological/Mouse+M-CSF+%2F+CSF-1+Protein/pm40398389-209-285-288
    Average 94 stars, based on 18 article reviews
    recombinant proteins m csf sinobiological - by Bioz Stars, 2026-10
    94/100 stars

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    Isolation:

    Article Title: PD-L1-CD80 interactions are required for intracellular signaling necessary for dendritic cell migration.
    Article Snippet: CD14+ PBMCs were isolated using a magnetic cell sorting separation protocol using liquid chromatography columns, anti–phycoerythrin (PE) microbeads (Miltenyi Biotec), and PE- conjugated CD14+ antibody (BioLegend, clone HCD14) according to the manufacturer’s instructions. .. Freshly isolated CD14+ cells were stimulated with recombinant human IL- 4 (250 U/ml, SinoBiological) and GM- CSF SinoBiological (100 U/ml) in RPMI 1640 containing 10% FBS and 1:100 each of Hepes (Corning), sodium pyruvate (Corning), nonessential amino acids (NEAA, Corning), 2- mercaptoethanol (Fisher Chemical), and penicillin- streptomycin (Corning) at days 0, 2, and 4, and, at day 5, moDCs were collected and used for downstream applications. .. For T cell stimulation, the cells were treated with phorbol 12- myristate 13- acetate and ionomycin (BioLegend) at 50 ng/ml for 6 hours before flow cytometric analysis.

    Recombinant:

    Article Title: PD-L1-CD80 interactions are required for intracellular signaling necessary for dendritic cell migration.
    Article Snippet: CD14+ PBMCs were isolated using a magnetic cell sorting separation protocol using liquid chromatography columns, anti–phycoerythrin (PE) microbeads (Miltenyi Biotec), and PE- conjugated CD14+ antibody (BioLegend, clone HCD14) according to the manufacturer’s instructions. .. Freshly isolated CD14+ cells were stimulated with recombinant human IL- 4 (250 U/ml, SinoBiological) and GM- CSF SinoBiological (100 U/ml) in RPMI 1640 containing 10% FBS and 1:100 each of Hepes (Corning), sodium pyruvate (Corning), nonessential amino acids (NEAA, Corning), 2- mercaptoethanol (Fisher Chemical), and penicillin- streptomycin (Corning) at days 0, 2, and 4, and, at day 5, moDCs were collected and used for downstream applications. .. For T cell stimulation, the cells were treated with phorbol 12- myristate 13- acetate and ionomycin (BioLegend) at 50 ng/ml for 6 hours before flow cytometric analysis.

    Clinical Proteomics:

    Article Title: PD-L1-CD80 interactions are required for intracellular signaling necessary for dendritic cell migration.
    Article Snippet: CD14+ PBMCs were isolated using a magnetic cell sorting separation protocol using liquid chromatography columns, anti–phycoerythrin (PE) microbeads (Miltenyi Biotec), and PE- conjugated CD14+ antibody (BioLegend, clone HCD14) according to the manufacturer’s instructions. .. Freshly isolated CD14+ cells were stimulated with recombinant human IL- 4 (250 U/ml, SinoBiological) and GM- CSF SinoBiological (100 U/ml) in RPMI 1640 containing 10% FBS and 1:100 each of Hepes (Corning), sodium pyruvate (Corning), nonessential amino acids (NEAA, Corning), 2- mercaptoethanol (Fisher Chemical), and penicillin- streptomycin (Corning) at days 0, 2, and 4, and, at day 5, moDCs were collected and used for downstream applications. .. For T cell stimulation, the cells were treated with phorbol 12- myristate 13- acetate and ionomycin (BioLegend) at 50 ng/ml for 6 hours before flow cytometric analysis.



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